light-colored objects (like white) reflects most of the light that strikes it, therefore appearing brighter than dark-colored objects (like brown, gray, and black) which absorbs most of the light striking it, making the object more "dense" in color, appearing darker.
Using the low power objective you can see not only the specimen but it also includes its surrounding therefore these parts not occupied by the specimen will allow more light on the field of the microscope so it is brighter while using the HPO as you might nave observed the lens is near the specimen and it focuses on certain parts of your specimen so whole part of it is pointed towards area covered by the specimen so less light is able to pass through therefore it is darker
light-colored objects (like white) reflects most of the light that strikes it, therefore appearing brighter than dark-colored objects (like brown, gray, and black) which absorbs most of the light striking it, making the object more "dense" in color, appearing darker.
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